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CyFlow™ CD4 PE

CyFlow™ CD4 PE
Antigen: CD4
Alternative Name: Leu3a, T4
Clone: GK1.5
Application: Flow cytometry
Format/Fluorochrome: PE
Laser: Blue, Green, Yellow
Target Species: Mouse
Field of Interest: Immunophenotyping, MHC
Species of Origin: Rat
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgG2b
Order number: CR188745

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Concentration 0.5 mg/ml Quantity 0.1 mg Volume 0.2 ml Immunogen Mouse CTL clone... more
CyFlow™ CD4 PE
Concentration0.5 mg/ml
Quantity0.1 mg
Volume0.2 ml
ImmunogenMouse CTL clone V4 cells
SpecifityThe rat monoclonal antibody GK1.5 recognizes an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
Background InformationCD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 3·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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