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CyFlow™ CD25 PE

CyFlow™ CD25 PE
Application: Flow cytometry
Excitation Maximum: 496 nm, 565 nm
Alternative Name: IL2Rα, p55, TAC
Antigen: CD25
Isotype: IgG1
Emission Maximum: 576 nm
Laser: Blue, Green, Yellow
Clonality: monoclonal
Format/Fluorochrome: PE
Field of Interest: Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Target Species: Human
Clone: MEM-181
Order number: AQ996615
220,00  EUR *

Prices net excl. VAT, plus applicable shipping costs

Delivery time appr. 3-5 workdays

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HLDA Workshop HLDA VI—WS Code NL N-L024 Quantity 100 tests Volume 2.0 mL Immunogen... more
Produktinformationen zu: "CyFlow™ CD25 PE"
HLDA WorkshopHLDA VI—WS Code NL N-L024
Quantity100 tests
Volume2.0 mL
ImmunogenPHA-activated peripheral blood leucocytes
Background InformationCD25 (IL2Rα, Tac) is a ligand-binding α subunit of interleukin 2 receptor (IL2R). Together with β and γ subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Safety Data Sheet

Technical Data Sheet

Certificate Of Analysis

ECDoC

Instruction for Use

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* All prices are quoted net of the statutory value-added tax and shipping costs and possibly delivery charges, if not otherwise described

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