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CyFlow™ CD34 Biotin

CyFlow™ CD34 Biotin
Antigen: CD34
Clone: 4H11[APG]
Application: Flow cytometry
Format/Fluorochrome: Biotin
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: BJ289364

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

 200.00 EUR*

Prices net excl. VAT, plus applicable shipping costs

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*1
*1 Offer valid until 31/10/2017
HLDA Workshop HLDA VI—WS Code M MA58 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD34 Biotin
HLDA WorkshopHLDA VI—WS Code M MA58
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1
ImmunogenPermanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia
Background InformationCD34 (Mucosialin) is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Krauter J, Hartl M, Hambach L, Kohlenberg A, Gunsilius E, Ganser A, Heil G: Receptor‑mediated endocytosis of CD34 on hematopoietic cells after stimulation with the monoclonal antibody anti‑HPCA‑1. J·Hematother·Stem·Cell·Res. 2001·Dec; 10(6):863‑71. <·PMID:·11798512·> | Dao MA, Arevalo J, Nolta JA: Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution. Blood. 2003·Jan·1; 101(1):112‑8. <·PMID:·12589631·> | Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL‑2, which is required for CD(4+)CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J·Leukoc·Biol. 2005·Sep; 78(3):725‑35. <·PMID:·16000396·> | Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Gulati S, Luthra PM: Three‑dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk‑L. Stem·Cells·Dev. 2005·Oct; 14(5):470‑7. <·PMID:·16305332·> | Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Luthra PM: Hematopoietic stem cell antigen CD34: role in adhesion or homing. Stem·Cells·Dev. 2006·Jun; 15(3):305‑13. <·PMID:·16846369·> | Elknerová K, Lacinová Z, Soucek J, Marinov I, Stöckbauer P: Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines. Neoplasma. 2007; 54(4):311‑20. <·PMID:·17822321·>