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CyFlow™ CD43 Alexa Fluor™ 700

CyFlow™ CD43 Alexa Fluor™ 700
Antigen: CD43
Alternative Name: Leukosialin, sialophorin
Clone: MEM-59
Application: Flow cytometry
Format/Fluorochrome: Alexa Fluor™ 700
Laser: Red
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 723 nm
Excitation Maximum: 702 nm
Isotype: IgG1
Order number: AV871619

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 0.4 ml Immunogen Human T lymphocytes Specifity The... more
CyFlow™ CD43 Alexa Fluor™ 700
Quantity100 tests
Volume0.4 ml
ImmunogenHuman T lymphocytes
SpecifityThe mouse monoclonal antibody MEM-59 recognizes neuraminidase-sensitive epitope on CD43 antigen, a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.
Background InformationCD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin, CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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