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CyFlow™ CD45 Alexa Fluor™ 488

CyFlow™ CD45 Alexa Fluor™ 488
Antigen: CD45
Alternative Name: B220, LCA, T200
Clone: MEM-28
Application: Flow cytometry
Format/Fluorochrome: Alexa Fluor™ 488
Laser: Blue
Target Species: Human
Field of Interest: Immunophenotyping, Phosphorylation
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 519 nm
Excitation Maximum: 495 nm
Isotype: IgG1
Order number: AK948230

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 0.4 ml Immunogen Human thymocytes and T lymphocytes... more
CyFlow™ CD45 Alexa Fluor™ 488
Quantity100 tests
Volume0.4 ml
ImmunogenHuman thymocytes and T lymphocytes
SpecifityThe mouse monoclonal antibody MEM-28 recognizes all alternative forms of human CD45 antigen, a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
Background InformationCD45 (LCA; leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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