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CyFlow™ CD58 APC

CyFlow™ CD58 APC
Antigen: CD58
Alternative Name: LFA-3
Clone: MEM-63
Application: Flow cytometry
Format/Fluorochrome: APC
Laser: Red
Target Species: Human, Pig
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Isotype: IgG1
Order number: AX001125

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 1 ml Immunogen NALM-6 human pre-B cell line Specifity... more
CyFlow™ CD58 APC
Quantity100 tests
Volume1 ml
ImmunogenNALM-6 human pre-B cell line
SpecifityThe mouse monoclonal antibody MEM-63 recognizes CD58 antigen, a 40-70 kDa glycoprotein distributed over many tissues, leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.
Background InformationCD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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