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CyFlow™ CD63 PerCP

CyFlow™ CD63 PerCP
Antigen: CD63
Alternative Name: LAMP-3, LIMP, MLA1
Clone: MEM-259
Application: Flow cytometry
Format/Fluorochrome: PerCP
Laser: Blue, Green
Target Species: Human
Field of Interest: Endocytosis, Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 678 nm
Excitation Maximum: 482 to 490 nm
Isotype: IgG1
Order number: BP806651

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

 230.00 EUR*

Prices net excl. VAT, plus applicable shipping costs

Quantity 100 tests Volume 1 ml Immunogen HPB-ALL T cell line Specifity The mouse... more
CyFlow™ CD63 PerCP
Quantity100 tests
Volume1 ml
ImmunogenHPB-ALL T cell line
SpecifityThe mouse monoclonal antibody MEM-259 recognizes CD63 antigen, a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
Background InformationCD63 (LAMP-3; lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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