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CyFlow™ CD71 APC-Cy7

CyFlow™ CD71 APC-Cy7
Antigen: CD71
Alternative Name: T9, TFR, TRFR
Clone: MEM-75
Application: Flow cytometry
Format/Fluorochrome: APC-Cy7
Laser: Red
Target Species: Human
Field of Interest: Endocytosis, Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 780 nm
Excitation Maximum: 650 nm, 750 nm
Isotype: IgG1
Order number: BZ847039

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

 270.00 EUR*

Prices net excl. VAT, plus applicable shipping costs

Quantity 100 tests Volume 0.4 ml Immunogen NALM-6 human pre-B cell line Specifity... more
CyFlow™ CD71 APC-Cy7
Quantity100 tests
Volume0.4 ml
ImmunogenNALM-6 human pre-B cell line
SpecifityThe mouse monoclonal antibody MEM-75 recognizes CD71 antigen, a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-75 does not block binding of transferrin to the receptor.
Background InformationCD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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