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CyFlow™ CD80 APC

CyFlow™ CD80 APC
Antigen: CD80
Alternative Name: B7- B7-1, BB1
Clone: MEM-233
Application: Flow cytometry
Format/Fluorochrome: APC
Laser: Red
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Isotype: IgG1
Order number: CZ682530

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

 245.00 EUR*

Prices net excl. VAT, plus applicable shipping costs

Quantity 100 tests Volume 1 ml Immunogen Extracellular domain of human CD80 fused to... more
CyFlow™ CD80 APC
Quantity100 tests
Volume1 ml
ImmunogenExtracellular domain of human CD80 fused to human IgG1(Fc)
SpecifityThe mouse monoclonal antibody MEM-233 recognizes CD80 antigen, a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Background InformationCD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CD152 (CTLA-4). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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