CyFlow™ CD45 APC-Cy7

Antigen:	CD45
Alternative Name:	B220, LCA, T200
Clone:	MEM-28
Application:	Flow cytometry
Format/Fluorochrome:	APC-Cy7
Laser:	Red
Target Species:	Human
Field of Interest:	Immunophenotyping, Phosphorylation
Species of Origin:	Mouse
Regulatory Status:	RUO
Clonality:	monoclonal
Emission Maximum:	780 nm
Excitation Maximum:	650 nm, 750 nm
Isotype:	IgG1
Order number:	AB648275

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

270,00 EUR*

Prices net excl. VAT, plus applicable shipping costs

Quantity 100 tests Volume 0.4 Immunogen Human thymocytes and T lymphocytes... more

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CyFlow™ CD45 APC-Cy7

Quantity	100 tests
Volume	0.4
Immunogen	Human thymocytes and T lymphocytes
Background Information	CD45 (LCA; leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Usage	The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage Buffer	The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
Storage	Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
Stability	Do not use after expiration date stamped on vial label.

Specific References

| McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·> | Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I: Monoclonal antibodies against human leucocyte antigens (II); Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18‑kDa antigen (MEM‑43) and a novel antigen of restricted expression (MEM‑74). Folia·Biol·(Praha). 1988; 34(1):23‑34. <·PMID:·2968928·> | Koene HR, Kleijer M, Algra J, Roos D, von dem Borne AE, de Haas M: Fc gammaRIIIa‑158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa‑48L/R/H phenotype. Blood. 1997·Aug·1; 90(3):1109‑14. <·PMID:·9242542·> | Bhunia AK, Arai T, Bulkley G, Chatterjee S: Lactosylceramide mediates tumor necrosis factor‑alpha‑induced intercellular adhesion molecule‑1 (ICAM‑1 expression and the adhesion of neutrophil in human umbilical vein endothelial cells. J·Biol·Chem. 1998·Dec·18; 273(51):34349‑57. <·PMID:·9852101·> | Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43‑mediated apoptosis of TF‑1 cells: Roles of transcription Daxx expression, and adhesion molecules. J·Biol·Chem. 2002·Mar·8; 277(10):7955‑61. <·PMID:·11773067·> | Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ: Combinations of CD45 isoforms are crucial for immune function and disease. J·Immunol. 2006·Mar·15; 176(6):3417‑25. <·PMID:·16517710·> | Cortez-Gonzalez X, Sidney J, Adotevi O, Sette A, Millard F, Lemonnier F, Langlade-Demoyen P, Zanetti M: Immunogenic HLA‑B7‑restricted peptides of hTRT. Int·Immunol. 2006·Dec; 18(12):1707‑18. <·PMID:·17077179·> | Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H: Role for CD47‑SIRPalpha signaling in xenograft rejection by macrophages. Proc·Natl·Acad·Sci·USA. 2007·Mar·20; 104(12):5062‑6. <·PMID:·17360380·> | Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single‑molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int·Immunol. 2007·May; 19(5):675‑84. <·PMID:·17446208·> | Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem·J. 2007·Sep·1; 406(2):333‑41. <·PMID:·17497959·> | Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2008·Feb; 13(2):197‑212. <·PMID:·18157742·> | McCormack E, Mujić M, Osdal T, Bruserud Ø , Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time‑domain imaging of acute myeloid leukemia. Blood. 2013·Feb·14; 121(7):e34‑42. <·PMID:·23243270·> | McCormack E, Mujić M, Osdal T, Bruserud Ø , Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time‑domain imaging of acute myeloid leukemia. Blood. 2013·Feb·14; 121(7):e34‑42. <·PMID:·23243270·>

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Sysmex Flow Cytometry Germany!