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CyFlow™ Lck Alexa Fluor™ 647

CyFlow™ Lck Alexa Fluor™ 647
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Laser: Red
Excitation Maximum: 650 nm
Isotype: IgG1
Format/Fluorochrome: Alexa Fluor™ 647
Emission Maximum: 665 nm
Field of Interest: Phosphorylation
Antigen: Lck
Clone: LCK-01
Order number: BG039735
330,00  EUR *

Prices net excl. VAT, plus applicable shipping costs

Delivery time appr. 3-5 workdays

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
Produktinformationen zu: "CyFlow™ Lck Alexa Fluor™ 647"
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenPeptide corresponding to amino acids 22-36 in the sequence of human Lck
Background InformationLck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its ζ subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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* All prices are quoted net of the statutory value-added tax and shipping costs and possibly delivery charges, if not otherwise described

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