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CyFlow™ IgG2a Alexa Fluor™ 488

CyFlow™ IgG2a Alexa Fluor™ 488
Clonality: monoclonal
Regulatory Status: RUO
Laser: Blue
Source Species: Mouse
Isotype: IgG2a
Format/Fluorochrome: Alexa Fluor™ 488
Excitation Maximum: 495 nm
Emission Maximum: 519 nm
Application: Flow cytometry (Isotype control)
Field of Interest: Isotype controls
Antigen: IgG2a Isotype Control
Clone: MOPC-173
Order number: BF508817

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

220,00  EUR*

Prices net excl. VAT, plus applicable shipping costs

Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
CyFlow™ IgG2a Alexa Fluor™ 488
Concentration Unitmg/mL
Concentration0,1
Quantity0.1 mg
Volume1.0 mL
ImmunogenThe transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice
Background InformationThe specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μ/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
UsageThe reagent is designed as an isotype control for Flow Cytometry analysis. To establish the amount of non-specific antibody binding, match the concentration of the correct isotype to the recommended working concentration of the antigen-specific antibody. If the background signal of the isotype control is too high (usually when working antibody concentrations are above 10·µg/ml of incubation mixture), change the experimental conditions to reduce the background.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Baumal R, Scharff MD: Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone. J·Immunol. 1976·Jan; 116(1):65‑74. <·PMID:·812916·> | Fougereau M, Bourgois A, de Preval C, Rocca-Serra J, Schiff C: The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications. Ann·Immunol·(Paris). 1976·Sep-Oct; 127(5):607‑31. <·PMID:·984731·> | Gupta V, Gylling A, Alonso JL, Sugimori T, Ianakiev P, Xiong JP, Arnaout MA: The beta‑tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18. Blood. 2007·Apr·15; 109(8):3513‑20. <·PMID:·17170130·> | Khoddami V, Cairns BR: Transcriptome‑wide target profiling of RNA cytosine methyltransferases using the mechanism‑based enrichment procedure Aza‑IP. Nat·Protoc. 2014·Feb; 9(2):337‑61. <·PMID:·24434802·>