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CyFlow™ CD45 Pacific Orange™

CyFlow™ CD45 Pacific Orange™
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping
Isotype: IgG1
Laser: Violet
Format/Fluorochrome: Pacific Orange™
Excitation Maximum: 400 nm
Emission Maximum: 551 nm
Antigen: CD45
Alternative Name: B220, LCA, T200
Clone: 2D1
Order number: AN249588
1,00  EUR *

Prices net excl. VAT, plus applicable shipping costs

Delivery time appr. 3-5 workdays

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HLDA Workshop HLDA III – WS Code 792 Quantity 100 tests Volume 0.4 mL Immunogen... more
Produktinformationen zu: "CyFlow™ CD45 Pacific Orange™"
HLDA WorkshopHLDA III – WS Code 792
Quantity100 tests
Volume0.4 mL
ImmunogenHuman peripheral blood mononuclear cells (PBMC)
Background InformationCD45 (LCA; leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
UsageThe reagent is designed for flow cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Safety Data Sheet

Technical Data Sheet

Instruction for Use

ECDoC

Certificate Of Analysis

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* All prices are quoted net of the statutory value-added tax and shipping costs and possibly delivery charges, if not otherwise described

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