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CyFlow™ TRBC-1 FITC

CyFlow™ TRBC-1 FITC
Application: Flow cytometry
Clonality: monoclonal
Format/Fluorochrome: FITC
Target Species: Human
Regulatory Status: RUO
Laser: Blue
Excitation Maximum: 490 to 495 nm
Emission Maximum: 518 nm
Source Species: Mouse
Field of Interest: Immunophenotyping
Antigen: TRBC-1
Alternative Name: TCRCB1, TRBC1
Clone: JOVI.1
Isotype: IgG2a kappa
Order number: BH960323
220,00  EUR *

Prices net excl. VAT, plus applicable shipping costs

Delivery time appr. 3-5 workdays

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Quantity 100 tests Volume 1.0 mL Immunogen Thymus, spleen, and mesenteric lymph... more
Produktinformationen zu: "CyFlow™ TRBC-1 FITC"

Quantity100 tests
Volume1.0 mL
ImmunogenThymus, spleen, and mesenteric lymph nodes isolated from a mouse transgenic for human TCR Vbeta3Cbeta1
Background InformationAlpha-beta T cell receptors (TCRs) are antigen specific receptors, which are essential to the immune response and are present on the cell surface of T lymphocytes. They recognize peptide-loaded major histocompatibility complexes (pMHCs), that are displayed by antigen presenting cells (APCs). Binding of alpha-beta TCR to pMHC initiates TCR-CD3 clustering on the cell surface and intracellular activation of LCK, that phosphorylates the ITAM motifs of CD3gamma, CD3delta, CD3epsilon and CD3zeta, enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium signaling, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NFkappaB (NF-kB) pathways, leading to the mobilization of transcription factors, that are critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire is generated by V-D-J-C rearrangements. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MHC restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TCRs with the pMHCs shapes TCR structural and functional avidity.
UsageThe reagent is designed for flow cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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* All prices are quoted net of the statutory value-added tax and shipping costs and possibly delivery charges, if not otherwise described

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