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CyFlow™ CD54 FITC

CyFlow™ CD54 FITC
Antigen: CD54
Alternative Name: ICAM-1
Clone: 1H4
Application: Flow cytometry
Format/Fluorochrome: FITC
Laser: Blue
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Isotype: IgG2b
Order number: BQ603568

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

 180.00 EUR*

Prices net excl. VAT, plus applicable shipping costs

Attention:

Order 2, get the lower priced for free

*1
*1 Offer valid until 31/10/2017
Quantity 100 tests Volume 2 Immunogen Raji cells and spleen cells fused with NS1... more
CyFlow™ CD54 FITC
Quantity100 tests
Volume2
ImmunogenRaji cells and spleen cells fused with NS1 cells
Background InformationCD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Boyd AW, Wawryk SO, Burns GF, Fecondo JV: Intercellular adhesion molecule 1 (ICAM‑1) has a central role in cell‑cell contact‑mediated immune mechanisms. Proc·Natl·Acad·Sci·USA. 1988·May; 85(9):3095‑9. <·PMID:·3362863·> | Boyd AW, Dunn SM, Fecondo JV, Culvenor JG, Duhrsen U, Burns GF, Wawryk SO: Regulation of expression of a human intercellular adhesion molecule (ICAM‑1) during lymphohematopoietic differentiation. Blood. 1989·May·15; 73(7):1896‑903. <·PMID:·2469503·> | Springer TA: Adhesion receptors of the immune system. Nature. 1990·Aug·2; 346(6283):425‑34. <·PMID:·1974032·> | Ockenhouse CF, Betageri R, Springer TA, Staunton DE: Plasmodium falciparum‑infected erythrocytes bind ICAM‑1 at a site distinct from LFA‑1, Mac‑1, and human rhinovirus. Cell. 1992·Jan·10; 68(1):63‑9. <·PMID:·1346257·> | Williams DT, Chaudhry Y, Goodfellow IG, Lea S, Evans DJ: Interactions of decay‑accelerating factor (DAF) with haemagglutinating human enteroviruses: utilizing variation in primate DAF to map virus binding sites. J·Gen·Virol. 2004·Mar; 85(3):731‑8. <·PMID:·14993659·>